- Journal
- JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
- Année
- 2010
- Volume
- 878
- Numéro
- 28
- Pages
- 2826-2830
- Mois
- OCT 15
- DOI
- 10.1016/j.jchromb.2010.08.036
Abstract
Arginase is an enzyme which plays a role in pathophysiology such as hypertension. Here we demonstrated for the first time the direct implication of pressure and OH degrees radical formation on the arginase activity via a novel analytical procedure. Pressure increased arginase activity in the range 12-52 bars. Activation by OH degrees radical showed a hyperbolic response. The OH degrees radicals produced were significantly inhibited by sulfasalazine (SAZ) and the inhibition of OH degrees radicals parallels the inhibition of arginase activity. (C) 2010 Elsevier B.V. All rights reserved.