2025 · RESTORATIVE DENTISTRY AND ENDODONTICS

In vitro experimental study comparing continuous and intermittent irrigation protocols: influence of sodium hypochlorite volume and contact time on tissue dissolution

Iandolo, Alfredo, Abdellatif, Dina, Mancino, Davide, Rolin, Gwenael, Coussens, Camille, Louvrier, Aurelian, Belladonna, Felipe G., Euvrard, Edouard, da Silva, Emmanuel Joao Nogueira Leal

Journal
RESTORATIVE DENTISTRY AND ENDODONTICS
Année
2025
Volume
50
Numéro
4
Article
e36
Mois
NOV
DOI
10.5395/rde.2025.50.e36

Abstract

Objectives: This study aimed to evaluate whether continuous irrigation with larger volumes or allowing sodium hypo-chlorite (NaOCl) resting time is more critical for pulp tissue dissolution using a controlled artificial root canal system. Methods: A three-dimensional printed artificial root canal with a lateral canal in the apical third was fabricated. Standardized bovine pulp tissue specimens were inserted, and three irrigation protocols were tested: group A (continuous NaOCl irrigation at 1 mL/min via syringe pump), group B (intermittent NaOCl irrigation with 0.1 mL and a 3-minute resting period), and group C (control, saline irrigation). The time for complete dissolution and the total NaOCl volume were recorded. Results: Complete dissolution occurred in groups A and B, with significant differences in NaOCl volume and time (p < 0.05). In group A, complete dissolution was consistently observed after the 6th irrigation cycle, corresponding to a total NaOCl volume of 6.0 +/- 0.66 mL per test. The average time required for complete dissolution in this group was 6 +/- 0.66 minutes. In group B, complete dissolution occurred after the 4th cycle, with a total NaOCl volume of 0.4 +/- 0.06 mL per test and a mean dissolution time of 12.6 +/- 1.8 minutes. Conclusions: NaOCl volume and exposure time significantly influence pulp tissue dissolution.

Lire l'article complet