2024 · JOURNAL OF THE PAKISTAN MEDICAL ASSOCIATION

Molecular identification of Hypomyces chrysospermus mycoparasitic fungus isolated from mushrooms in a local market of Iraq

Hamdan, Noor Talib

Journal
JOURNAL OF THE PAKISTAN MEDICAL ASSOCIATION
Année
2024
Volume
74
Numéro
10, 8
Pages
398-401
Mois
OCT
DOI
10.47391/JPMA-BAGH-16-90

Abstract

In this study, mycoparasitic fungus was identified by using the rDNA internal transcribed spacer barcode marker, i.e. ITS rDNA gene. Amplicons were sequenced and identified by NCBI - BLAST (Basic local alignment search tool). The BLAST results revealed that NOOR strain matched 100% with accession numbers [MH854754.1]. The phylogenetic tree shows close relationship between NOOR strain and H. chrysospermus [MH854754-1], H. chrysospermus [MZ389105-1], H. chrysospermus [MK6053271], H. chrysospermus [MT595227-1], H. [EU816370-1], H. chrysospermus [MG685885-1]. The Iraqi isolate was recorded as NOOR strain in the National Centre for Biotechnology Information for the first time in Iraq.

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