- Journal
- TALANTA
- Année
- 2011
- Volume
- 84
- Numéro
- 3
- Pages
- 632-637
- Mois
- MAY 15
- DOI
- 10.1016/j.talanta.2011.01.038
Abstract
The current work describes the elaboration of a simple, sensitive and reliable beta-casein modified glass beads, for the detection and quantification of its specific antibody anti beta-casein. This is an elementary receptor without electronic part, developed by grafting glass bead surface with the antigenic beta-casein via 3-aminopropyltriethoxy silane and then glutaraldehyde as cross-linker. The whole is realized by a classical process, called in two steps and in mild conditions where chemical protocol is optimized for beta-casein use. The detection and quantification of the specific reaction antibody-receptor is carried out by the technique of the second antibody labeled with horse radish peroxidase (HRP). Our receptor can detect the beta-casein antibody present in the serum at dilutions up to a factor 10(7) in strong ionic strength medium. The same antibody of the same serum and in the same conditions can be detected by ELISA test at dilutions up to a factor 10(5). The whole test, after our receptor realization, takes about 5 h. (C) 2011 Elsevier B.V. All rights reserved.